# 5-Amino-1MQ — dosage, half-life & reference guide | PepVault

Source: https://pepvault.app/peptides/5-amino-1mq

Last updated: 2026-09-26

[Library](https://pepvault.app/peptides) [Metabolic & GLP-1](https://pepvault.app/peptides#metabolic) reviewed September 2026

# 5-Amino-1MQ Dosage, half-life & reference guide

Also known as 5-A-1MQ · 5Amino1MQ · 5-Amino-1-methylquinolinium

A small molecule (not a peptide) that blocks the NNMT enzyme in fat cells, which raises NAD+ and pushes fat cells toward burning energy. In obese mice it reduced weight and fat without cutting food intake.

- **Studied range:** 50–100 mg
- **How often:** Daily
- **Half-life:** Unknown
- **Evidence:** Emerging
- **Route:** Oral

Studied in research — not a recommendation.

[5-Amino-1MQ dose guide](https://pepvault.app/lab/dosage/5-amino-1mq)

## How it works.

NNMT (Nicotinamide N-methyltransferase) inhibitor that blocks NNMT activity in fat cells. In cultured fat cells it raises NAD+ and SAM levels; in obese mice it cut body weight and fat without reducing food intake, and with a reduced-calorie diet lean mass made up a larger share of body weight. No human data.

**Commonly reported:** Nothing to feel, though some describe easier fat loss alongside a deficit or a GLP-1. There are no human studies, and the mouse work used injections rather than oral doses.

## Where 5-Amino-1MQ works.

- ### Appetite & metabolism

  **In animals.** In obese mice it cut body weight and fat without reducing food intake.
- ### Energy & longevity

  **In the lab.** In cultured fat cells it raises NAD+ and SAM levels.

An area lights for what a peptide is used for and what people report, with the evidence beside it. It isn’t a promise that it works there.

## Week by week.

What to expect in the first 8 weeks

1. Day 1–28 Animal

   ### Quiet compound, slow signal

   5-Amino-1MQ doesn't announce itself. Commonly reported: no appetite change, no energy spike, no obvious sensation. What mouse data shows is a slightly better body-composition response to the same diet, especially in a deficit, so give it the full month before judging.

   The science

   5-Amino-1-methylquinolinium is a selective NNMT (nicotinamide N-methyltransferase) inhibitor. In cultured fat cells, blocking NNMT raised NAD+ and SAM and reduced fat synthesis; this pushes fat cells toward burning energy (SIRT1 involvement is proposed, and fat oxidation wasn't measured). Neelakantan et al. 2018: obese mice injected three times daily lost about 5% of body weight over 11 days while controls gained about 1.4%, with food intake unchanged.

   Sources

   - Neelakantan et al. (2018)

   Updated Sep 2026
2. Day 28–84 Animal

   ### Recomp in a deficit

   The rodent data's clearest signal is fat loss without eating less; paired with a diet, lean mass made up a larger share of body weight. If you're using this with a cut, this is the window to watch scale, waist and grip strength together.

   The science

   NNMT inhibition shifts fat-cell metabolism toward energy expenditure and away from storage. In a follow-up mouse study, adding the inhibitor to a reduced-calorie diet raised the lean-to-body-weight ratio compared with diet alone (Sampson 2021). Human data is essentially nonexistent — we're extrapolating.

   Sources

   - Neelakantan et al. (2018)
   - Sampson et al. (2021)

   Updated Sep 2026
3. Any day Animal

   ### What 5-Amino-1MQ is built on

   Interesting mechanism, positive rodent data, zero completed human trials. Treat as an early-stage research compound you're testing on yourself. Small molecule, not a peptide — included because it's commonly tracked alongside metabolic peptide protocols.

   The science

   Developed originally as a tool compound to study NNMT biology. Rodent pharmacokinetics are only partly published, and human pharmacokinetics are not published. Research-use-only status in the US. Quality and purity of community-sourced material is an additional concern — verify your source.

   Sources

   - Neelakantan et al. (2018)
   - Sampson et al. (2021)

   Updated Sep 2026
4. With Semaglutide, Tirzepatide and Retatrutide Community

   ### Layered onto a GLP-1 cut

   Common community pattern: 5-Amino-1MQ layered onto an ongoing GLP-1 protocol. The GLP-1 drives the calorie deficit while 5-Amino-1MQ helps protect lean mass from the fat-cell side (a mouse finding). Commonly reported: some find recomposition a bit easier; others notice no change.

   The science

   GLP-1 agonists drive weight loss primarily via appetite reduction; 5-Amino-1MQ acts peripherally at fat cells on NNMT/NAD+ signaling (SIRT1 involvement is proposed). By mechanism, additive rather than synergistic. No head-to-head or combination trial exists.

   Sources

   - Neelakantan et al. (2018)

   Updated Sep 2026

### After week 8

1. Day 84–168 Animal

   ### Cycle decision point

   Most community use lands in the 8–12 week range, but honestly: no chronic human data exists, so any endpoint is arbitrary. Treat this as a reassessment checkpoint — did body composition shift, did metabolic markers move? A 2–3 month break before a second run is the conservative default.

   The science

   No chronic human dosing data exists. NNMT has different roles in fat, liver and brain (Roberti 2021 review), so long-term blocking could have different effects in each, one argument for cycling; the cycle length itself is community convention.

   Sources

   - Roberti et al. (2021)

   Updated Sep 2026

Day 1 is the day you start it.

## A typical run.

### Run notes

- **Cycle:** Very limited data. Some run 8–12 weeks then cycle off. Preclinical compound — approach conservatively. Community practice — opinions vary. Not a clinical recommendation.
- **Timing:** Oral daily. Timing not well established.
- **Route:** Oral
- **Stacks:** NMN, Semaglutide

## Safety.

### Common side effects

- Limited human data
- Generally well tolerated in early research

## Goes with.

- [NMN](https://pepvault.app/peptides/nmn)
- [Semaglutide](https://pepvault.app/peptides/semaglutide)

## Questions people ask.

### What is 5-Amino-1MQ?

A small molecule (not a peptide) that blocks the NNMT enzyme in fat cells, which raises NAD+ and pushes fat cells toward burning energy. In obese mice it reduced weight and fat without cutting food intake.

### How is 5-Amino-1MQ dosed in research?

The research range for 5-Amino-1MQ is 50–100 mg, daily. Studied in research — not a recommendation.

### How long does 5-Amino-1MQ last?

The half-life of 5-Amino-1MQ is unknown.

### What do people report on 5-Amino-1MQ?

Commonly reported on 5-Amino-1MQ: nothing to feel, though some describe easier fat loss alongside a deficit or a GLP-1. There are no human studies, and the mouse work used injections rather than oral doses.

### Is 5-Amino-1MQ safe?

The research basis for 5-Amino-1MQ is emerging: primarily preclinical / limited human data. Common side effects in the library: limited human data and generally well tolerated in early research.

### What does 5-Amino-1MQ stack with?

Common stacks for 5-Amino-1MQ: NMN and Semaglutide.

## Research.

The literature

### Search the latest research

[PubMed](https://pubmed.ncbi.nlm.nih.gov/?term=5-Amino-1MQ) [Google Scholar](https://scholar.google.com/scholar?q=5-Amino-1MQ)

## Reference.

- **Cadence:** Daily
- **Half-life:** Unknown
- **Research basis:** Emerging Primarily preclinical / limited human data

## More metabolic & glp-1 peptides

- [SLU-PP-332 250–1000 mcg](https://pepvault.app/peptides/slu-pp-332)
- [Oxytocin 10–40 IU](https://pepvault.app/peptides/oxytocin)
- [Semaglutide (oral) 1.5–25 mg](https://pepvault.app/peptides/semaglutide-oral)
- [Tirzepatide 2.5–15 mg](https://pepvault.app/peptides/tirzepatide)
- [Retatrutide 1–12 mg](https://pepvault.app/peptides/retatrutide)
- [Liraglutide 0.6–3 mg](https://pepvault.app/peptides/liraglutide)

## Free tools

- [5-Amino-1MQ dose guide](https://pepvault.app/lab/dosage/5-amino-1mq)
- [Peptide Sky (3D)](https://pepvault.app/lab/sky)
- [Peptide tier list](https://pepvault.app/lab/tiers)
- [Reconstitution calculator](https://pepvault.app/tools/reconstitution-calculator)
- [Half-life & active level](https://pepvault.app/tools/peptide-half-life-calculator)
- [GLP-1 titration planner](https://pepvault.app/tools/glp1-titration-calculator)

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Reviewed September 2026 [How we check the library](https://pepvault.app/about#library)

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PepVault is a private research and tracking tool, not medical advice. The information here is reference material from published literature and community use — always verify it and discuss any protocol with a qualified clinician before changing it.

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